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Image Search Results
Journal: Oncology reports
Article Title: PLAU is associated with cell migration and invasion and is regulated by transcription factor YY1 in cervical cancer.
doi: 10.3892/or.2022.8462
Figure Lengend Snippet: Figure 1. PLAU is overexpressed in cervical cancer. (A) PLAU expression in normal tissues and cervical cancer tissues based on the UALCAN data base. (B) Expression of PLAU in 16 cervical cancer tissues and matched tumor‑adjacent normal tissues was detected using reverse transcription‑quan titative PCR. *P<0.05. PLAU, plasminogen activator urokinase; TCGA, The Cancer Genome Atlas.
Article Snippet: The lentiviral vectors (Lv)‐small hairpin RNA (shRNA) based on a 3rd
Techniques: Expressing
Journal: Oncology reports
Article Title: PLAU is associated with cell migration and invasion and is regulated by transcription factor YY1 in cervical cancer.
doi: 10.3892/or.2022.8462
Figure Lengend Snippet: Figure 2. Knockdown of PLAU inhibits cell proliferation, migration and invasion of cervical cancer. (A) PLAU mRNA expression in HeLa and HT3 cells infected with Lv‑shCon or Lv‑shPLAU. (B and C) The proliferation of (B) HeLa and (C) HT3 cells with Lv‑shCon or Lv‑shPLAU infection. (D) The migration and invasion of HeLa cells with Lv‑shCon or Lv‑shPLAU infection. (E) Counting of migratory and invasive HeLa cells. (F) Cell migration and invasion of HT3 cells with Lv‑shCon or Lv‑shPLAU infection. (G) Counting of migratory and invasive HT3 cells. (H and I) Expression of cyclin D1, MMP‑9, and E‑cadherin in (H) HeLa and (I) HT3 cells with Lv‑shCon or Lv‑shPLAU infection. *P<0.05. PLAU, plasminogen activator urokinase; Lv, lentiviral; sh‑, short hairpin.
Article Snippet: The lentiviral vectors (Lv)‐small hairpin RNA (shRNA) based on a 3rd
Techniques: Knockdown, Migration, Expressing, Infection
Journal: Oncology reports
Article Title: PLAU is associated with cell migration and invasion and is regulated by transcription factor YY1 in cervical cancer.
doi: 10.3892/or.2022.8462
Figure Lengend Snippet: Figure 3. Identification of the PLAU core promoter. (A) Based on the pGL3‑PLAU, a series of deletion mutants of the PLAU promoter (‑1500/+18, ‑1300/+18, ‑1100/+18, ‑900/+18, ‑700/+18, ‑500/+18, ‑300/+18) were constructed and transfected into HeLa cells to measure the luciferase activity. Left panel, the ideograph of the deletion mutants of the PLAU promoter; right panel, relative luciferase activity of each mutant. (B) Left panel, ideograph of the PLAU core promoter; right panel, relative luciferase activity. *P<0.05 vs. the ‑1500/+18. PLAU, plasminogen activator urokinase; luc, luciferase.
Article Snippet: The lentiviral vectors (Lv)‐small hairpin RNA (shRNA) based on a 3rd
Techniques: Construct, Transfection, Luciferase, Activity Assay, Mutagenesis
Journal: Oncology reports
Article Title: PLAU is associated with cell migration and invasion and is regulated by transcription factor YY1 in cervical cancer.
doi: 10.3892/or.2022.8462
Figure Lengend Snippet: Figure 4. YY1 regulates PLAU expression at the transcriptional level. (A) A total of 8 transcriptional factors were predicted to bind to the PLAU core promoter according to PROMO software. (B) YY1 expression in normal and cervical cancer tissues based on the UALCAN database. (C) Expression of YY1 in 16 cervical cancer tissues and matched tumor‑adjacent normal tissues was detected using reverse transcription‑quantitative PCR. (D) Correlation of PLAU to YY1 expression in cervical cancers. (E) The migration and invasion of HeLa cells with Lv‑shCon or Lv‑shYY1 infection. (F) Counting of migratory and invasive HeLa cells. (G) mRNA expression levels of cyclin D1, MMP‑9, and E‑cadherin in HeLa cells with Lv‑shCon or Lv‑shYY1 infection. (H) The migration and invasion of HT3 cells with Lv‑shCon or Lv‑shYY1 infection. (I) Counting of migratory and invasive HT3 cells. (J) mRNA expression levels of cyclin D1, MMP‑9 and E‑cadherin in HT3 cells with Lv‑shCon or Lv‑shYY1 infection. (K) PLAU mRNA expression in HeLa cells with Lv‑shCon or Lv‑shYY1 infection. (L) PLAU core promoter activity in HeLa cells with Lv‑shCon or Lv‑shYY1 infection. *P<0.05. YY1, transcription factor YinYang 1; PLAU, plasminogen activator urokinase; Lv, lentiviral; sh‑, short hairpin.
Article Snippet: The lentiviral vectors (Lv)‐small hairpin RNA (shRNA) based on a 3rd
Techniques: Expressing, Software, Migration, Infection, Activity Assay
Journal: Oncology reports
Article Title: PLAU is associated with cell migration and invasion and is regulated by transcription factor YY1 in cervical cancer.
doi: 10.3892/or.2022.8462
Figure Lengend Snippet: Figure 5. Identification of the binding site of YY1 on the PLAU promoter. (A) Site A and Site B were mutated separately or double‑mutated. (B) Luciferase activity of the PLAU promoter with separate Site A and Site B mutation, and with double mutation of Site A and Site B were measured in HeLa cells with Lv‑shCon or Lv‑shYY1 infection. *P<0.05. YY1, transcrip tion factor YinYang 1; PLAU, plasminogen activator urokinase; Lv, lentiviral; sh‑, short hairpin.
Article Snippet: The lentiviral vectors (Lv)‐small hairpin RNA (shRNA) based on a 3rd
Techniques: Binding Assay, Luciferase, Activity Assay, Mutagenesis, Infection